Dextranase of Streptococcus mutans.
نویسندگان
چکیده
منابع مشابه
Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.
Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = ...
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A more sensitive test agar was developed to detect oral microbes with relatively low dextranase activity and to identify dextranase-negative mutants. Several oral streptococci that had previously been scored as dextranase negative readily decolorized the new, blue dextran-containing medium. To assess whether dextranase plays a role in glucan synthesis by oral streptococci, various glucan synthe...
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The colonization and accumulation of Streptococcus mutans are influenced by various factors in the oral cavity, such as nutrition and hygiene conditions of the host, salivary components, cleaning power and salivary flow and characteristics related with microbial virulence factors. Among these virulence factors, the ability to synthesize glucan of adhesion, glucan-binding proteins, lactic acid a...
متن کاملEffect of dextranase on the extracellular polysaccharide synthesis of Streptococcus mutans; chemical and scanning electron microscopy studies.
A dextranase preparation (AD17) partially purified from a culture liquor of Spicaria violacea strain IFO 6120 significantly inhibited the formation of artifcial dental plaque on a steel wire or on an extracted tooth surface. Changes in the surface morphology of Streptococcus mutans cells due to AD17 action were studied using scanning electron microscopy. S. mutans cells grown in 5% sucrose-cont...
متن کاملEvaluation of the effect of Kandovan propolis against Streptococcus Mutans
BACKGROUND AND AIM: In recent years, propolis has been introduced as one the most efficient agents against cariogenic bacteria. However, due to the lack of data on the propolis collected from Kandovan (Easthern Azarbaijan, Iran), this study was designed to investigate the effect of this type of propolis on Streptococcus mutans (S. mutans).METHODS: In this experimental study, the ethanolic extra...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1998
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.53.435